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KMID : 0881720170320020083
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 2 p.83 ~ p.88
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Development of Disposable Immunosensors for Rapid Determination of Sildenafil and Vardenafil in Functional Foods
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Vijayaraj Kathiresan
Lee Jun-Hyuck
Kim Hyung-Sik
Chang Seung-Cheol
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Abstract
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We introduced disposable amperometric immunosensors for the detection of Sildenafil and Vardenafil (SDF/VDF) based on screen printed carbon electrodes. The developed immunosensors were used as a non-competitive sandwich-type enzyme immunoassay with a horseradish peroxidase label. The sensors were constructed on screen printed carbon electrodes by the simple electrochemical deposition of a reduced graphene oxide and chitosan (ErGO-CS) composite. To evaluate the sensing chemistry and optimize the sensor characteristics, a series of electrochemical experiments were carried out including electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The sensors showed a linear response to SDF/VDF concentrations in a range from 100 pg/mL to 300 ng/mL. The lower detection limit was calculated to be 55 pg/mL, the sensitivity was calculated to be 1.02 μAng/mL/cm2, and the sensor performance exhibited good reproducibility with a relative standard deviation (RSD) of 7.1%. The proposed sensing chemistry strategy and the sensor format can be used as a simple, cost-effective, and feasible method for the in-field analysis of SDF/VDF in functional or health supplement food samples.
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KEYWORD
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Disposable sensor , Functional foods , Immunosensor , Sildenafil/Vardenafil
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